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The cDNA sequence of human NAMPT was amplified by PCR from a pGex-6p-3-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, University of Missouri). The PCR solutions were being digested and cloned right into a pET21a+ vector using NdeI and XhoI restriction enzymes. A degree mutation was launched by the quick modify web page-directed mutagenesis str